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Marker Proteins Molecular Weight (daltons) Distance Serum Album in (dimer) 132,0

Marker Proteins
Molecular Weight (daltons)
Distance
Serum Album in (dimer)
132,000
23mm
Serum Album in (monomer)
66,000
32mm
Ovalbumin in
43,000
39mm
Lysozyme
14,400
60mm
Unknown 1
?
26mm
Unknown 2
?
58mm
Rabbit Serum (albumin)
?
33mm
The Lab Handout is attached below as an example of how the Assignment is supposed to look.
Answer the Questions below. use the data and semi-log paper provided here to find out the size (MW) of unknown proteins 1 and 2 as well as the rabbit serum protein albumen. (?)
Use the data in the table above to answer these questions.
1. On semilog paper/graph paper provided below, plot the distance migrated by each standard protein as a function of molecular weight. Determine the molecular weight of the unknown proteins and the major protein found in rabbit serum (albumin) from this calibration curve.
2. Interferon is a protein of 25,000 daltons. How far would it have migrated, had it been included in this experiment?
3. The average amino acid residue in a protein has a molecular weight of 120 daltons Assuming that your unknowns are average, how many amino acid residues do they contain?

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