Report 1: Gram Stain due: _______________
You have been given 3 samples to run t
Report 1: Gram Stain due: _______________
You have been given 3 samples to run the gram stain. A control gram positive Staphylococcus aureus, a control gram negative Escherichia coli and an unknown.
Abstract: (4 points) Paragraph summary of the paper
Intro: central question and hypothesis, short background. 1-3 sentences (1 point)
Brief methods: What did you do? 1-3 sentences (1 point)
Results: What were the results? 1-3 sentences (1 point)
Conclusions: Summarized 1-3 sentences (1 point)
Introduction (Theory and background) (9 points) (Citations should be in the text in APA format)
Short history of the Gram stain. Role of the gram stain in medicine and microbiology. (2 points)
What are the major and minor parts of the two types of bacterial cell walls. What are the functions of those parts? (3 points) Go beyond what the textbook gives- will require outside sources and should be referenced
Describes what happens to the two types of bacterial cell walls (chemically speaking) at each step of the Gram stain. (4 points)
Why and What happens to each cell type when crystal violet is applied?
Why and What happens to each cell type as iodine is applied?
Why and What happens to each cell type as decolorizer/alcohol is applied?
Why and What happens to each cell type as safranin is applied?
Results (2 points) (in paragraph form)
Only include the microscopic observations (color, shape and grouping/arrangement: see chapter 3) Make sure to use technical terms.
If (not required) you include an image of your microscope results, remember to label the image with a figure number and a title. In addition, refer to the image in the text of the results with a description of the image.
Conclusions (3 points) (Citations should be in the text in APA format)
Briefly restate results and discuss how you categorize the microbes (controls and unknown) you viewed under the microscope?
Based on the category, what physical attributes (components of cell wall) are possessed by your controls and your unknown organism? How did your controls help you to identify these for your unknown?
Connect the ideas from the introduction section about medical significance and your unknown results.
